Nanotheranostics 2019; 3(1):113-119. doi:10.7150/ntno.30924

Research Paper

Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues

Mingmin Li2#, Jianzhen Wu1#, Mengtian Ma1, Zhichao Feng1, Ze Mi1, Pengfei Rong1✉, Dingbin Liu2 ✉

1. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
2. College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
# M. L. and J. W. contributed equally to this work.

Abstract

Surface-enhanced Raman spectroscopy (SERS) has proven a powerful tool for multiplex detection and imaging due to its narrow peak width and high sensitivity. However, conventional SERS reporters are limited to thiolated compounds, which have limitations such as chemical stability and spectral overlap. Here, we used alkyne- and nitrile-bearing molecules to directly fabricate a set of SERS tags for multiplex imaging. The alkyne and nitrile groups act as both the anchoring points to interact with gold nanoparticle (AuNP) surfaces and the reporters exhibiting strong and nonoverlapping signals in the cellular Raman-silent region. The SERS tags were subsequently modified with different antibodies for multicolor imaging of cancer cells and human breast cancer tissues. The reporters have a simple and readily accessible structure, hence providing new opportunities to prepare SERS nanoprobes

Keywords: surface-enhanced Raman spectroscopy (SERS), gold nanoparticles, multicolor imaging, background-free detection

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How to cite this article:
Li M, Wu J, Ma M, Feng Z, Mi Z, Rong P, Liu D. Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues. Nanotheranostics 2019; 3(1):113-119. doi:10.7150/ntno.30924. Available from http://www.ntno.org/v03p0113.htm