Nanotheranostics 2018; 2(2):144-156. doi:10.7150/ntno.22433

Research Paper

Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis

Yong Cheol Shin1*, Chuntae Kim2*, Su-Jin Song3, Seungwon Jun3, Chang-Seok Kim3, Suck Won Hong3, Suong-Hyu Hyon4, Dong-Wook Han3,✉, Jin-Woo Oh2,✉

1. Research Center for Energy Convergence Technology, Pusan National University, Busan 46241, Republic of Korea
2. Department of Nanofusion Technology, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Republic of Korea
3. Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Republic of Korea
4. Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan
*Yong Cheol Shin and Chuntae Kim contributed equally to this work.

Abstract

Recently, there have been tremendous efforts to develop the biofunctional scaffolds by incorporating various biochemical factors. In the present study, we fabricated poly(lactic-co-glycolic acid) (PLGA) nanofiber sheets decorated with graphene oxide (GO) and RGD peptide. The decoration of GO and RGD peptide was readily achieved by using RGD peptide-displaying M13 bacteriophage (RGD-M13 phage) and electrospinning. Furthermore, the aligned GO-decorated PLGA/RGD peptide (GO-PLGA/RGD) ternary nanofiber sheets were prepared by magnetic field-assisted electrospinning, and their potentials as bifunctional scaffolds for facilitating myogenesis were explored. We characterized the physicochemical and mechanical properties of the sheets by scanning electron microscopy, Raman spectroscopy, contact angle measurement, and tensile test. In addition, the C2C12 skeletal myoblasts were cultured on the aligned GO-PLGA/RGD nanofiber sheets, and their cellular behaviors, including initial attachment, proliferation and myogenic differentiation, were evaluated. Our results revealed that the GO-PLGA/RGD nanofiber sheets had suitable physicochemical and mechanical properties for supporting cell growth, and could significantly promote the spontaneous myogenic differentiation of C2C12 skeletal myoblasts. Moreover, it was revealed that the myogenic differentiation was further accelerated on the aligned GO-PLGA/RGD nanofiber sheets due to the synergistic effects of RGD peptide, GO and aligned nanofiber structure. Therefore, , it is suggested that the aligned GO-PLGA/RGD ternary nanofiber sheets are one of the most promising approaches for facilitating myogenesis and promoting skeletal tissue regeneration.

Keywords: poly(lactic-co-glycolic acid), graphene oxide, RGD peptide, M13 bacteriophage, magnetic field-assisted electrospinning, myogenesis.

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How to cite this article:
Shin YC, Kim C, Song SJ, Jun S, Kim CS, Hong SW, Hyon SH, Han DW, Oh JW. Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis. Nanotheranostics 2018; 2(2):144-156. doi:10.7150/ntno.22433. Available from http://www.ntno.org/v02p0144.htm