Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Nanotheranostics 2024; 8(3):298-311. doi:10.7150/ntno.93064 This issue Cite

Research Paper

Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis

Ilaria Zanolla1, Martina Trentini2, Elena Tiengo2, Federica Zanotti2, Tommaso Pusceddu2, Andrea Rubini3, Giuseppe Rubini4, Francesca Brugnoli4, Danilo Licastro5, Marco Debortoli5, Lucia Gemma Delogu6, Letizia Ferroni6, Luca Lovatti2, Barbara Zavan2✉

1. Department of Medical Sciences, University of Ferrara, 44121, Ferrara, Italy.
2. Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy.
3. University of Bologna, 40126, Bologna, Italy.
4. Ultravet Diagnostic, 40017, San Giovanni in Persiceto, Italy.
5. AREA Science Park, 34149, Trieste, Italy.
6. Department of Biomedical Sciences, University of Padua, 35122, Padua, Italy.
7. Maria Cecilia Hospital, GVM Care & Research, 48033, Cotignola, Italy.

✉ Corresponding author: Barbara Zavan, Email: barbara.zavanit.

Citation:
Zanolla I, Trentini M, Tiengo E, Zanotti F, Pusceddu T, Rubini A, Rubini G, Brugnoli F, Licastro D, Debortoli M, Delogu LG, Ferroni L, Lovatti L, Zavan B. Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. Nanotheranostics 2024; 8(3):298-311. doi:10.7150/ntno.93064. https://www.ntno.org/v08p0298.htm
Other styles

File import instruction

Abstract

Graphic abstract

Exosomes are nanosized extracellular vesicles secreted by all cell types, including canine adipose-derived stem cells (cADSCs). By mediating intercellular communication, exosomes modulate the biology of adjacent and distant cells by transferring their cargo. In the present work after isolation and characterization of exosomes derived from canine adipose tissue, we treated the same canine donors affected by hepatopathies with the previously isolated exosomes. We hypothesize that cADSC-sourced miRNAs are among the factors responsible for a regenerative and anti-inflammatory effect in the treatment of hepatopathies in dogs, providing the clinical veterinary field with an effective and innovative cell-free therapy. Exosomes were isolated and characterized for size, distribution, surface markers, and for their miRNomic cargo by microRNA sequencing. 295 dogs affected with hepatopathies were treated and followed up for 6 months to keep track of their biochemical marker levels. Results confirmed that exosomes derived from cADSCs exhibited an average diameter of 91 nm, and positivity to 8 known exosome markers. The administration of exosomes to dogs affected by liver-associated inflammatory pathologies resulted in the recovery of the animal alongside the normalization of biochemical parameters of kidney function. In conclusion, cADSCs-derived exosomes are a promising therapeutic tool for treating inflammatory disorders in animal companions.

Keywords: extracellular nanovesicles, exosomes, miRNA, liver diseases, regenerative medicine.

Citation styles

APA
Zanolla, I., Trentini, M., Tiengo, E., Zanotti, F., Pusceddu, T., Rubini, A., Rubini, G., Brugnoli, F., Licastro, D., Debortoli, M., Delogu, L.G., Ferroni, L., Lovatti, L., Zavan, B. (2024). Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. Nanotheranostics, 8(3), 298-311. https://doi.org/10.7150/ntno.93064.

ACS
Zanolla, I.; Trentini, M.; Tiengo, E.; Zanotti, F.; Pusceddu, T.; Rubini, A.; Rubini, G.; Brugnoli, F.; Licastro, D.; Debortoli, M.; Delogu, L.G.; Ferroni, L.; Lovatti, L.; Zavan, B. Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. Nanotheranostics 2024, 8 (3), 298-311. DOI: 10.7150/ntno.93064.

NLM
Zanolla I, Trentini M, Tiengo E, Zanotti F, Pusceddu T, Rubini A, Rubini G, Brugnoli F, Licastro D, Debortoli M, Delogu LG, Ferroni L, Lovatti L, Zavan B. Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. Nanotheranostics 2024; 8(3):298-311. doi:10.7150/ntno.93064. https://www.ntno.org/v08p0298.htm

CSE
Zanolla I, Trentini M, Tiengo E, Zanotti F, Pusceddu T, Rubini A, Rubini G, Brugnoli F, Licastro D, Debortoli M, Delogu LG, Ferroni L, Lovatti L, Zavan B. 2024. Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. Nanotheranostics. 8(3):298-311.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use