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Nanotheranostics 2017; 1(2):178-185. doi:10.7150/ntno.18720 This issue Cite

Research Paper

Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria

Jinjoo Kim^{1, 3*}, Hansol Kim^{2, 4*}, Ji Ho Park^{2, 4✉}, Sangyong Jon^{1, 3✉}

1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
2. Department of Bio & Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
3. KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
4. KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea.
* These authors contributed equally to this work.

✉ Corresponding authors: jihoparkac.kr; syjonac.kr

Abstract

The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In the photo-PCR system, poly(enthylene glycol)-modified gold nanorods (PEG-GNRs), used as a heat generator, are added into the PCR mixture, which is subsequently periodically irradiated with a 808-nm laser to create thermal cycling. Photo-PCR was able to significantly reduce overall thermal cycling time by integrating bacterial cell lysis and DNA amplification into a single step. Furthermore, when combined with KAPA2G fast polymerase and cooling system, the entire process of bacterial genomic DNA extraction and amplification was further shortened, highlighting the potential of photo-PCR for use in a portable, POC diagnostic system.

Keywords: bacterial detection, diagnosis, gold nanorod, polymerase chain reaction (PCR), photothermal heating.

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