Nanotheranostics 2023; 7(2):152-166. doi:10.7150/ntno.79050 This issue Cite

Research Paper

Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer

Georges Minassian1, Esther Ghanem1, Roland El Hage2, Kamil Rahme1,3✉

1. Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University - Louaize, Lebanon.
2. Faculty of Sciences, Fanar Campus, Chemistry & Physics Department, and Doctoral School, Lebanese University, Beirut, Lebanon.
3. School of Chemistry & AMBER Centre, University College Cork, Cork, T12 YN60, Ireland.

Citation:
Minassian G, Ghanem E, Hage RE, Rahme K. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics 2023; 7(2):152-166. doi:10.7150/ntno.79050. https://www.ntno.org/v07p0152.htm
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Abstract

Graphic abstract

Dendrigraft Poly-L-Lysine (d-PLL) coated gold nanoparticles (AuNPs) were synthesized by reducing Tetrachloroauric acid with ascorbic acid in the presence of d-PLL. AuNPs-d-PLL formed a stable colloidal solution that absorbs light at a maximum wavelength (λmax) centered at 570 nm as demonstrated by UV-visible (UV-Vis) spectroscopy. From Scanning Electron Microscopy (SEM) analysis, AuNPs-d-PLL were spherical in shape with a mean diameter of 128 ± 47 nm. Dynamic Light scattering (DLS) analysis of the colloidal solution exhibited one size distribution with a hydrodynamic diameter of about 131 nm (size distribution by intensity). Zeta potential (ξ) measurements revealed positively charged AuNPs-d-PLL with ξ about 32 mV, an indicator of high stability in an aqueous solution. The AuNPs-d-PLL was successfully modified with either thiolated poly (ethylene glycol) SH-PEG-OCH3 (Mw 5400 g mol-1) or folic acid-modified thiolated poly (ethylene glycol) SH-PEG-FA of similar molecular weight as demonstrated via DLS and Zeta potential measurements. Complexation of PEGylated AuNPs-d-PLL with siRNA was confirmed by DLS and gel electrophoresis. Finally, we analyzed the functionalization of our nanocomplexes with folic acid via targeted cellular uptake to prostate cancer cells using flow cytometry and LSM imaging. Our findings implicate the broader applicability of folate-PEGylated AuNPs in siRNA-based therapeutics against prostate cancer and perhaps other types of cancer.

Keywords: gold nanoparticles, polymers, stabilization, siRNA delivery, cancer


Citation styles

APA
Minassian, G., Ghanem, E., Hage, R.E., Rahme, K. (2023). Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics, 7(2), 152-166. https://doi.org/10.7150/ntno.79050.

ACS
Minassian, G.; Ghanem, E.; Hage, R.E.; Rahme, K. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics 2023, 7 (2), 152-166. DOI: 10.7150/ntno.79050.

NLM
Minassian G, Ghanem E, Hage RE, Rahme K. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics 2023; 7(2):152-166. doi:10.7150/ntno.79050. https://www.ntno.org/v07p0152.htm

CSE
Minassian G, Ghanem E, Hage RE, Rahme K. 2023. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics. 7(2):152-166.

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