Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer

Minassian, Georges; Ghanem, Esther; Hage, Roland El; Rahme, Kamil

doi:10.7150/ntno.79050

Theranostics

International Journal of Biological Sciences

International Journal of Medical Sciences

Journal of Cancer

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Nanotheranostics 2023; 7(2):152-166. doi:10.7150/ntno.79050 This issue Cite

Research Paper

Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer

Georges Minassian¹, Esther Ghanem¹, Roland El Hage², Kamil Rahme^1,3✉

1. Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University - Louaize, Lebanon.
2. Faculty of Sciences, Fanar Campus, Chemistry & Physics Department, and Doctoral School, Lebanese University, Beirut, Lebanon.
3. School of Chemistry & AMBER Centre, University College Cork, Cork, T12 YN60, Ireland.

Citation:

Minassian G, Ghanem E, Hage RE, Rahme K. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics 2023; 7(2):152-166. doi:10.7150/ntno.79050. https://www.ntno.org/v07p0152.htm

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Abstract

Dendrigraft Poly-L-Lysine (d-PLL) coated gold nanoparticles (AuNPs) were synthesized by reducing Tetrachloroauric acid with ascorbic acid in the presence of d-PLL. AuNPs-d-PLL formed a stable colloidal solution that absorbs light at a maximum wavelength (λ_max) centered at 570 nm as demonstrated by UV-visible (UV-Vis) spectroscopy. From Scanning Electron Microscopy (SEM) analysis, AuNPs-d-PLL were spherical in shape with a mean diameter of 128 ± 47 nm. Dynamic Light scattering (DLS) analysis of the colloidal solution exhibited one size distribution with a hydrodynamic diameter of about 131 nm (size distribution by intensity). Zeta potential (ξ) measurements revealed positively charged AuNPs-d-PLL with ξ about 32 mV, an indicator of high stability in an aqueous solution. The AuNPs-d-PLL was successfully modified with either thiolated poly (ethylene glycol) SH-PEG-OCH₃ (M_w 5400 g mol^-1) or folic acid-modified thiolated poly (ethylene glycol) SH-PEG-FA of similar molecular weight as demonstrated via DLS and Zeta potential measurements. Complexation of PEGylated AuNPs-d-PLL with siRNA was confirmed by DLS and gel electrophoresis. Finally, we analyzed the functionalization of our nanocomplexes with folic acid via targeted cellular uptake to prostate cancer cells using flow cytometry and LSM imaging. Our findings implicate the broader applicability of folate-PEGylated AuNPs in siRNA-based therapeutics against prostate cancer and perhaps other types of cancer.

Keywords: gold nanoparticles, polymers, stabilization, siRNA delivery, cancer

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APA

Minassian, G., Ghanem, E., Hage, R.E., Rahme, K. (2023). Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics, 7(2), 152-166. https://doi.org/10.7150/ntno.79050.

ACS

Minassian, G.; Ghanem, E.; Hage, R.E.; Rahme, K. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics 2023, 7 (2), 152-166. DOI: 10.7150/ntno.79050.

NLM

CSE

Minassian G, Ghanem E, Hage RE, Rahme K. 2023. Gold Nanoparticles Conjugated with Dendrigraft Poly-L-lysine and Folate-Targeted Poly(ethylene glycol) for siRNA Delivery to Prostate cancer. Nanotheranostics. 7(2):152-166.

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